Isolation, culture, and transplantation of muscle satellite cells

N Motohashi, Y Asakura, A Asakura - JoVE (Journal of Visualized …, 2014 - jove.com
Muscle satellite cells are a stem cell population required for postnatal skeletal muscle
development and regeneration, accounting for 2-5% of sublaminal nuclei in muscle fibers. In
adult muscle, satellite cells are normally mitotically quiescent. Following injury, however,
satellite cells initiate cellular proliferation to produce myoblasts, their progenies, to mediate
the regeneration of muscle. Transplantation of satellite cell-derived myoblasts has been
widely studied as a possible therapy for several regenerative diseases including muscular …