Development and implementation of an international proficiency testing program for a neutralizing antibody assay for HIV-1 in TZM-bl cells
CA Todd, KM Greene, X Yu, DA Ozaki, H Gao… - Journal of …, 2012 - Elsevier
Recent advances in assay technology have led to major improvements in how HIV-1
neutralizing antibodies are measured. A luciferase reporter gene assay performed in TZM-bl
(JC53bl-13) cells has been optimized and validated. Because this assay has been adopted
by multiple laboratories worldwide, an external proficiency testing program was developed
to ensure data equivalency across laboratories performing this neutralizing antibody assay
for HIV/AIDS vaccine clinical trials. The program was optimized by conducting three …
neutralizing antibodies are measured. A luciferase reporter gene assay performed in TZM-bl
(JC53bl-13) cells has been optimized and validated. Because this assay has been adopted
by multiple laboratories worldwide, an external proficiency testing program was developed
to ensure data equivalency across laboratories performing this neutralizing antibody assay
for HIV/AIDS vaccine clinical trials. The program was optimized by conducting three …