[HTML][HTML] Generation of N-ethyl-N-nitrosourea (ENU) diabetes models in mice demonstrates genotype-specific action of glucokinase activators

D Fenner, S Odili, HK Hong, Y Kobayashi… - Journal of Biological …, 2011 - ASBMB
We performed genome-wide mutagenesis in C57BL/6J mice using N-ethyl-N-nitrosourea to
identify mutations causing high blood glucose early in life and to produce new animal
models of diabetes. Of a total of 13 new lines confirmed by heritability testing, we identified
two semi-dominant pedigrees with novel missense mutations (Gck K140E and Gck P417R)
in the gene encoding glucokinase (Gck), the mammalian glucose sensor that is mutated in
human maturity onset diabetes of the young type 2 and the target of emerging anti …