Multiplex genome engineering using CRISPR/Cas systems

L Cong, FA Ran, D Cox, S Lin, R Barretto, N Habib… - Science, 2013 - science.org
Science, 2013science.org
Functional elucidation of causal genetic variants and elements requires precise genome
editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short
palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided
site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and
demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage
at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a …
Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a nicking enzyme to facilitate homology-directed repair with minimal mutagenic activity. Lastly, multiple guide sequences can be encoded into a single CRISPR array to enable simultaneous editing of several sites within the mammalian genome, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
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