Mutagenesis and Mapping of a Mouse Gene, Clock, Essential for Circadian Behavior
MH Vitaterna, DP King, AM Chang, JM Kornhauser… - Science, 1994 - science.org
Science, 1994•science.org
In a search for genes that regulate circadian rhythms in mammals, the progeny of mice
treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A
semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of
rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion
of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU
mutagenesis combined with the ability to clone murine genes by map position provides a …
treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A
semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of
rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion
of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU
mutagenesis combined with the ability to clone murine genes by map position provides a …
In a search for genes that regulate circadian rhythms in mammals, the progeny of mice treated with N-ethyl-N-nitrosourea (ENU) were screened for circadian clock mutations. A semidominant mutation, Clock, that lengthens circadian period and abolishes persistence of rhythmicity was identified. Clock segregated as a single gene that mapped to the midportion of mouse chromosome 5, a region syntenic to human chromosome 4. The power of ENU mutagenesis combined with the ability to clone murine genes by map position provides a generally applicable approach to study complex behavior in mammals.
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