[HTML][HTML] In vivo migration and function of transferred HIV-1-specific cytotoxic T cells

SJ Brodie, DA Lewinsohn, BK Patterson, D Jiyamapa… - Nature medicine, 1999 - nature.com
SJ Brodie, DA Lewinsohn, BK Patterson, D Jiyamapa, J Krieger, L Corey, PD Greenberg
Nature medicine, 1999nature.com
The persistence of HIV replication in infected individuals may reflect an inadequate host HIV-
specific CD8+ cytotoxic T lymphocyte (CTL) response. The functional activity of HIV-specific
CTLs and the ability of these effector cells to migrate in vivo to sites of infection was directly
assessed by expanding autologous HIV-1 Gag-specific CD8+ CTL clones in vitro and
adoptively transferring these CTLs to HIV-infected individuals. The transferred CTLs retained
lytic function in vivo, accumulated adjacent to HIV-infected cells in lymph nodes and …
Abstract
The persistence of HIV replication in infected individuals may reflect an inadequate host HIV-specific CD8+ cytotoxic T lymphocyte (CTL) response. The functional activity of HIV-specific CTLs and the ability of these effector cells to migrate in vivo to sites of infection was directly assessed by expanding autologous HIV-1 Gag-specific CD8+ CTL clones in vitro and adoptively transferring these CTLs to HIV-infected individuals. The transferred CTLs retained lytic function in vivo, accumulated adjacent to HIV-infected cells in lymph nodes and transiently reduced the levels of circulating productively infected CD4+ T cells. These results provide direct evidence that HIV-specific CTLs target sites of HIV replication and mediate antiviral activity, and indicate that the development of immunotherapeutic approaches to sustain a strong CTL response to HIV may be a useful adjunct to treatment of HIV infection.
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