[HTML][HTML] Assembly dynamics and stoichiometry of the apoptosis signal-regulating kinase (ASK) signalosome in response to electrophile stress

JD Federspiel, SG Codreanu, AM Palubinsky… - Molecular & Cellular …, 2016 - ASBMB
Apoptosis signal-regulating kinase 1 (ASK1) is a key sensor kinase in the mitogen-activated
protein kinase pathway that transduces cellular responses to oxidants and electrophiles.
ASK1 is regulated by a large, dynamic multiprotein signalosome complex, potentially
including over 90 reported ASK1-interacting proteins. We employed both shotgun and
targeted mass spectrometry assays to catalogue the ASK1 protein-protein interactions in
HEK-293 cells treated with the prototypical lipid electrophile 4-hydroxy-2-nonenal (HNE) …