METHOD Inadequacies of existing techniques for measurement of NEFA made a new
method desirable. The proce-dure described below is rapid and simple with about 3 per cent
coefficient of variation on replicate analysis and a sensitivity sufficient for accurate titration of
fatty acids in 1 cc. of plasma. It appears to be quite specific for non-esterified fatty acids, at
least with respect to other organic acids likely to enter plasma, but it cannot be regarded as
absolutely reliable. For the present all that can be claimed is that NEFA are quantitatively …