Imprint of human cytomegalovirus infection on the NK cell receptor repertoire

M Gumá, A Angulo, C Vilches, N Gómez-Lozano… - Blood, 2004 - ashpublications.org
M Gumá, A Angulo, C Vilches, N Gómez-Lozano, N Malats, M López-Botet
Blood, 2004ashpublications.org
Expression of the activating CD94/NKG2C killer lectin-like receptor (KLR) specific for HLA-E
was analyzed in peripheral blood lymphocytes (PBLs) from healthy adult blood donors; the
expression of other natural killer (NK) cell receptors (ie, CD94/NKG2A, KIR, CD85j, CD161,
NKp46, NKp30, and NKG2D) was also studied. Human cytomegalovirus (HCMV) infection
as well as the HLA-E and killer immunoglobulin-like receptor (KIR) genotypes were
considered as potentially relevant variables associated with CD94/NKG2C expression. The …
Expression of the activating CD94/NKG2C killer lectin-like receptor (KLR) specific for HLA-E was analyzed in peripheral blood lymphocytes (PBLs) from healthy adult blood donors; the expression of other natural killer (NK) cell receptors (ie, CD94/NKG2A, KIR, CD85j, CD161, NKp46, NKp30, and NKG2D) was also studied. Human cytomegalovirus (HCMV) infection as well as the HLA-E and killer immunoglobulin-like receptor (KIR) genotypes were considered as potentially relevant variables associated with CD94/NKG2C expression. The proportion of NKG2C+ lymphocytes varied within a wide range (<0.1% to 22.1%), and a significant correlation (r = 0.83; P < .001) between NKG2C+ NK and T cells was noticed. The HLA-E genotype and the number of activating KIR genes of the donors were not significantly related to the percentage of NKG2C+ lymphocytes. By contrast, a positive serology for HCMV, but not for other herpesviruses (ie, Epstein-Barr and herpes simplex), turned out to be strongly associated (P < .001) with increased proportions of NKG2C+ NK and T cells. Remarkably, the CD94/NKG2C+ population expressed lower levels of natural cytotoxicity receptors (NCRs) (ie, NKp30, NKp46) and included higher proportions of KIR+ and CD85j+ cells than CD94/NKG2A+ cells. Altogether, these data support that HCMV infection selectively shapes the natural killer cell receptor (NKR) repertoire of NK and T cells from healthy carrier individuals.
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