Paracrine purinergic signaling determines lung endothelial nitric oxide production

R Kiefmann, MN Islam, J Lindert… - … of Physiology-Lung …, 2009 - journals.physiology.org
R Kiefmann, MN Islam, J Lindert, K Parthasarathi, J Bhattacharya
American Journal of Physiology-Lung Cellular and Molecular …, 2009journals.physiology.org
Although the vascular bed is a major source of nitric oxide (NO) production, factors
regulating the production remain unclear. We considered the role played by paracrine
signaling. Determinations by fluorescence microscopy in isolated, blood-perfused rat and
mouse lungs revealed that a brief lung expansion enhanced cytosolic Ca2+ (Ca2+ cyt)
oscillations in alveolar epithelial (AEC) and endothelial (EC) cells, and NO production in EC.
Furthermore, as assessed by a novel microlavage assay, alveolar ATP production …
Although the vascular bed is a major source of nitric oxide (NO) production, factors regulating the production remain unclear. We considered the role played by paracrine signaling. Determinations by fluorescence microscopy in isolated, blood-perfused rat and mouse lungs revealed that a brief lung expansion enhanced cytosolic Ca2+ (Ca2+cyt) oscillations in alveolar epithelial (AEC) and endothelial (EC) cells, and NO production in EC. Furthermore, as assessed by a novel microlavage assay, alveolar ATP production increased. Intra-alveolar microinfusion of the purinergic receptor antagonist, PPADS, and the nucleotide hydrolyzing enzyme, apyrase, each completely blocked the Ca2+cyt and NO responses in EC. Lung expansion induced Ca2+cyt oscillations in mice lacking the P2Y1, but not the P2Y2, purinergic receptors, which were located in the perivascular interstitium basolateral to AEC. Prolonged lung expansion instituted by mechanical ventilation at high tidal volume increased EC expression of nitrotyrosine, indicating development of nitrosative stress in lung microvessels. These findings reveal a novel mechanism in which mechanically induced purinergic signaling couples cross-compartmental Ca2+cyt oscillations to microvascular NO production.
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