Zinc-dependent activation of the plasma kinin-forming cascade by aggregated β amyloid protein
Y Shibayama, K Joseph, Y Nakazawa… - Clinical …, 1999 - Elsevier
β Amyloid proteins (Aβ) of 38, 40, and 42 amino acids long were assessed for their ability to
activate the plasma kinin-forming cascadein vitro. Incubation with a mixture of Factor XII
(Hageman Factor), prekallikrein, and high-molecular-weight kininogen (HK) led to
conversion of prekallikrein to kallikrein that was dependent on zinc ion. No activation
occurred if Factor XII was omitted. There was rapid generation of bradykinin equal to the
molar HK input indicating complete cleavage. Incubation of aggregated Aβ with diluted …
activate the plasma kinin-forming cascadein vitro. Incubation with a mixture of Factor XII
(Hageman Factor), prekallikrein, and high-molecular-weight kininogen (HK) led to
conversion of prekallikrein to kallikrein that was dependent on zinc ion. No activation
occurred if Factor XII was omitted. There was rapid generation of bradykinin equal to the
molar HK input indicating complete cleavage. Incubation of aggregated Aβ with diluted …