Metabolism of fructose in the small intestine I. The effect of fructose feeding on fructose transport and metabolism in rat small intestine
DA Mavrias, RJ Mayer - Biochimica et Biophysica Acta (BBA) …, 1973 - Elsevier
DA Mavrias, RJ Mayer
Biochimica et Biophysica Acta (BBA)-Biomembranes, 1973•ElsevierThe metabolism and transport of fructose in rat small intestine following fructose feeding for
up to 20 days has been measured. A preparation of villus epithelial cells was used for the
measurements. 2. In confirmation of the results of other workers fructose was poorly
metabolised in rat small intestine. Small conversion to lactate and glucose was observed. 3.
Fructose feeding had virtually no effect on the conversion of fructose to lactate or glucose. 4.
Fructose feeding for 3 days resulted in a significant increase (approx. 2-fold) in the activities …
up to 20 days has been measured. A preparation of villus epithelial cells was used for the
measurements. 2. In confirmation of the results of other workers fructose was poorly
metabolised in rat small intestine. Small conversion to lactate and glucose was observed. 3.
Fructose feeding had virtually no effect on the conversion of fructose to lactate or glucose. 4.
Fructose feeding for 3 days resulted in a significant increase (approx. 2-fold) in the activities …
Abstract
- 1.The metabolism and transport of fructose in rat small intestine following fructose feeding for up to 20 days has been measured. A preparation of villus epithelial cells was used for the measurements.
- 2.In confirmation of the results of other workers fructose was poorly metabolised in rat small intestine. Small conversion to lactate and glucose was observed.
- 3.Fructose feeding had virtually no effect on the conversion of fructose to lactate or glucose.
- 4.Fructose feeding for 3 days resulted in a significant increase (approx. 2-fold) in the activities of fructokinase (EC 2.7.1.1) and fructose-1-phosphate aldolase (EC 4.1.2.7) in rat small intestine whereas after fructose feeding for 15 days these enzyme activities were not significantly different from enzyme activities in the small intestine of animals fed on normal laboratory chow.
- 5.Measurements of the rate of fructose transport in segments of intestine taken from rats fed on normal laboratory chow or the fructose diet were made. The rate of fructose uptake in segments of intestine taken from animals fed for 3 days on the fructose diet was about twice that of animals fed on normal laboratory chow. The rate of fructose uptake was not further altered if segments were obtained from intestine of animals which had received the fructose diet for 15 days.
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