Metabolic intermediates in liver of rats given large amounts of fructose or dihydroxyacetone

HB Burch, P Max Jr, K Chyu, OH Lowry - Biochemical and Biophysical …, 1969 - Elsevier
HB Burch, P Max Jr, K Chyu, OH Lowry
Biochemical and Biophysical Research Communications, 1969Elsevier
The livers of rats given large injections of fructose or dihydroxyacetone were analyzed for
glucose, glycogen, and 14 related metabolites. Dihydroxyacetone produced a large
increase in glycogen and a modest increase in glucose and glucose-6-P. Fructose produced
no increase in glycogen but a large increase in glucose and glucose-6-P. Changes in
intermediates suggest in both cases diversion of part of the injected material into the
Embden-Meyerhof pathway. Fructose caused a profound drop in ATP, UTP and UDPG. The …
Abstract
The livers of rats given large injections of fructose or dihydroxyacetone were analyzed for glucose, glycogen, and 14 related metabolites. Dihydroxyacetone produced a large increase in glycogen and a modest increase in glucose and glucose-6-P. Fructose produced no increase in glycogen but a large increase in glucose and glucose-6-P. Changes in intermediates suggest in both cases diversion of part of the injected material into the Embden-Meyerhof pathway. Fructose caused a profound drop in ATP, UTP and UDPG. The fall in ATP is attributed to sequestration of phosphate into fructose-1-P, which increased to 18 mmole/kg. The decrease in ATP is believed to lead in turn to the fall in UTP and UDPG and the failure to deposit glycogen.
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