Constitutive activation of STAT5 by a point mutation in the SH2 domain
K Ariyoshi, T Nosaka, K Yamada, M Onishi… - Journal of Biological …, 2000 - jbc.org
We previously identified a constitutively active form of STAT (signal transducer and activator
of transcription) 5A by polymerase chain reaction-driven random mutagenesis followed by
retrovirus-mediated expression screening, which had two point mutations in the DNA-
binding and transcriptional activation domains, and was designated STAT5A1* 6. STAT5A1*
6 showed markedly elevated DNA binding and transactivation activities with stable tyrosine
phosphorylation and nuclear accumulation, and conferred autonomous cell growth on …
of transcription) 5A by polymerase chain reaction-driven random mutagenesis followed by
retrovirus-mediated expression screening, which had two point mutations in the DNA-
binding and transcriptional activation domains, and was designated STAT5A1* 6. STAT5A1*
6 showed markedly elevated DNA binding and transactivation activities with stable tyrosine
phosphorylation and nuclear accumulation, and conferred autonomous cell growth on …
