Enzymes involved in the metabolism of γ-hydroxybutyrate in SH-SY5Y cells: identification of an iron-dependent alcohol dehydrogenase ADHFe1

RC Lyon, SM Johnston, A Panopoulos, S Alzeer… - Chemico-biological …, 2009 - Elsevier
The metabolism of the endogenous metabolite γ-hydroxybutyrate (GHB) has been studied in
a human neuroblastoma cell line SH-SY5Y as a model for examining neuronal metabolism.
We show that GHB can be synthesized and released from these cells, indicating that
pathways for GHB synthesis and secretion are present. Activities for the major enzymes that
are involved in GHB metabolism are reported, and transcripts for AKR1A1, AKR7A2,
ALDH5A1 and GABA-T can be detected by RT-PCR. We also demonstrate the presence of …