Publisher Summary This chapter describes the use of DNA methyltransferase/endonuclease
enzyme combinations for megabase mapping of chromosomes. This chapter describes four
selected DNA methylase/endonuclease combinations that may be used for megabase
mapping of chromosome fragments in the size range from 100 to 2000 kilobases (kb),
paying special attention to features of these reactions that have sometimes proven
problematic. Accordingly, methyltransferase/endonuclease enzyme has proven necessary …