The magnitude of the spontaneous production of histamine-releasing factor (HRF) by lymphocytes in vitro correlates with the state of bronchial hyperreactivity in …

R Alam, P Kuna, J Rozniecki, B Kuzminska - Journal of allergy and clinical …, 1987 - Elsevier
In our previous studies we reported that lymphocytes from patients with asthma
spontaneously produce histamine-releasing factor (HRF) in vitro. In an effort to examine
whether spontaneous HRF production (SpHRF) by lymphocytes from patients with asthma is
related to the state of bronchial hyperreactivity (BHR), 20 patients with mild to severe asthma
were studied. Peripheral blood lymphocytes were cultured alone in a serum-free medium for
24 hours, and culture supernatant was assayed for HRF activity in two separate histamine …