Analyzing real-time PCR data by the comparative CT method

TD Schmittgen, KJ Livak - Nature protocols, 2008 - nature.com
TD Schmittgen, KJ Livak
Nature protocols, 2008nature.com
Two different methods of presenting quantitative gene expression exist: absolute and
relative quantification. Absolute quantification calculates the copy number of the gene
usually by relating the PCR signal to a standard curve. Relative gene expression presents
the data of the gene of interest relative to some calibrator or internal control gene. A widely
used method to present relative gene expression is the comparative CT method also
referred to as the 2-ΔΔ CT method. This protocol provides an overview of the comparative …
Abstract
Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene. A widely used method to present relative gene expression is the comparative CT method also referred to as the method. This protocol provides an overview of the comparative CT method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.
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