[HTML][HTML] H19 mRNA-like noncoding RNA promotes breast cancer cell proliferation through positive control by E2F1
N Berteaux, S Lottin, D Monté, S Pinte… - Journal of biological …, 2005 - ASBMB
The imprinted H19 gene has riboregulatory functions. We show here that H19 transcription
is up-regulated during the S-phase of growth-stimulated cells and that the H19 promoter is
activated by E2F1 in breast cancer cells. H19 repression by pRb and E2F6 confirms the
E2F1-dependent control of the H19 promoter. Consistently, we demonstrate by chromatin
immunoprecipitation assays that endogenous E2F1 is recruited to the H19 promoter in vivo.
The functionality of E2F promoter sites was further confirmed by gel shift and mutagenesis …
is up-regulated during the S-phase of growth-stimulated cells and that the H19 promoter is
activated by E2F1 in breast cancer cells. H19 repression by pRb and E2F6 confirms the
E2F1-dependent control of the H19 promoter. Consistently, we demonstrate by chromatin
immunoprecipitation assays that endogenous E2F1 is recruited to the H19 promoter in vivo.
The functionality of E2F promoter sites was further confirmed by gel shift and mutagenesis …