Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy

AS Nicot, A Toussaint, V Tosch, C Kretz… - Nature …, 2007 - nature.com
AS Nicot, A Toussaint, V Tosch, C Kretz, C Wallgren-Pettersson, E Iwarsson, H Kingston…
Nature genetics, 2007nature.com
Centronuclear myopathies are characterized by muscle weakness and abnormal
centralization of nuclei in muscle fibers not secondary to regeneration. The severe neonatal
X-linked form (myotubular myopathy) is due to mutations in the phosphoinositide
phosphatase myotubularin (MTM1), whereas mutations in dynamin 2 (DNM2) have been
found in some autosomal dominant cases. By direct sequencing of functional candidate
genes, we identified homozygous mutations in amphiphysin 2 (BIN1) in three families with …
Abstract
Centronuclear myopathies are characterized by muscle weakness and abnormal centralization of nuclei in muscle fibers not secondary to regeneration. The severe neonatal X-linked form (myotubular myopathy) is due to mutations in the phosphoinositide phosphatase myotubularin (MTM1), whereas mutations in dynamin 2 (DNM2) have been found in some autosomal dominant cases. By direct sequencing of functional candidate genes, we identified homozygous mutations in amphiphysin 2 (BIN1) in three families with autosomal recessive inheritance. Two missense mutations affecting the BAR (Bin1/amphiphysin/RVS167) domain disrupt its membrane tubulation properties in transfected cells, and a partial truncation of the C-terminal SH3 domain abrogates the interaction with DNM2 and its recruitment to the membrane tubules. Our results suggest that mutations in BIN1 cause centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic membranes, and that the functional interaction between BIN1 and DNM2 is necessary for normal muscle function and positioning of nuclei.
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