Building cells from their component parts will hinge upon our ability to reconstitute
biochemical compartmentalization and exchange between membrane-delimited organelles.
By contrast with our understanding of other cellular events, the mechanisms that govern
membrane trafficking has lagged because the presence of phospholipid bilayers
complicates the use of standard methods. This chapter describes in vitro methods for
purifying, reconstituting, and visualizing membrane remodeling activities directly by electron …