Molecular identification of an IgE-dependent histamine-releasing factor

SM MacDonald, T Rafnar, J Langdon, LM Lichtenstein - Science, 1995 - science.org
SM MacDonald, T Rafnar, J Langdon, LM Lichtenstein
Science, 1995science.org
An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by
lymphocytes of atopic children and present in biological fluids of allergic patients has been
identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse
protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine
release from the human basophils of a subpopulation of donors, and this release was
dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of …
An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by lymphocytes of atopic children and present in biological fluids of allergic patients has been identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine release from the human basophils of a subpopulation of donors, and this release was dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of recombinant and native HRF. HRF identifies a heterogeneity of IgE and is believed to play a prominent role in chronic allergic disease processes.
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