[HTML][HTML] Action of protein disulfide isomerase on proinsulin exit from endoplasmic reticulum of pancreatic β-cells
G Rajpal, I Schuiki, M Liu, A Volchuk, P Arvan - Journal of biological …, 2012 - ASBMB
For insulin synthesis, the proinsulin precursor is translated at the endoplasmic reticulum
(ER), folds to include its three native disulfide bonds, and is exported to secretory granules
for processing and secretion. Protein disulfide isomerase (PDI) has long been assumed to
assist proinsulin in this process. Herein we have examined the effect of PDI knockdown (PDI-
KD) in β-cells. The data establish that upon PDI-KD, oxidation of proinsulin to form native
disulfide bonds is unimpaired and in fact enhanced. This is accompanied by improved …
(ER), folds to include its three native disulfide bonds, and is exported to secretory granules
for processing and secretion. Protein disulfide isomerase (PDI) has long been assumed to
assist proinsulin in this process. Herein we have examined the effect of PDI knockdown (PDI-
KD) in β-cells. The data establish that upon PDI-KD, oxidation of proinsulin to form native
disulfide bonds is unimpaired and in fact enhanced. This is accompanied by improved …
