[HTML][HTML] Treg-mediated suppression of atherosclerosis requires MYD88 signaling in DCs

M Subramanian, E Thorp… - The Journal of clinical …, 2013 - Am Soc Clin Investig
The Journal of clinical investigation, 2013Am Soc Clin Investig
TLR activation on CD11c+ DCs triggers DC maturation, which is critical for T cell activation.
Given the expansion of CD11c+ DCs during the progression of atherosclerosis and the key
role of T cell activation in atherogenesis, we sought to understand the role of TLR signaling
in CD11c+ DCs in atherosclerosis. To this end, we used a mouse model in which a key TLR
adaptor involved in DC maturation, MYD88, is deleted in CD11c+ DCs. We transplanted
bone marrow containing Myd88-deficient CD11c+ DCs into Western diet–fed LDL receptor …
TLR activation on CD11c+ DCs triggers DC maturation, which is critical for T cell activation. Given the expansion of CD11c+ DCs during the progression of atherosclerosis and the key role of T cell activation in atherogenesis, we sought to understand the role of TLR signaling in CD11c+ DCs in atherosclerosis. To this end, we used a mouse model in which a key TLR adaptor involved in DC maturation, MYD88, is deleted in CD11c+ DCs. We transplanted bone marrow containing Myd88-deficient CD11c+ DCs into Western diet–fed LDL receptor knockout mice and found that the transplanted mice had decreased activation of effector T cells in the periphery as well as decreased infiltration of both effector T cells and Tregs in atherosclerotic lesions. Surprisingly, the net effect was an increase in atherosclerotic lesion size due to an increase in the content of myeloid-derived inflammatory cells. The mechanism involves increased lesional monocyte recruitment associated with loss of Treg-mediated suppression of MCP-1. Thus, the dominant effect of MYD88 signaling in CD11c+ DCs in the setting of atherosclerosis is to promote the development of atheroprotective Tregs. In the absence of MYD88 signaling in CD11c+ DCs, the loss of this protective Treg response trumps the loss of proatherogenic T effector cell activation.
The Journal of Clinical Investigation