Targeting DNA double-strand breaks with TAL effector nucleases
Genetics, 2010•academic.oup.com
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for
targeted mutagenesis. Here we report a new class of sequence-specific nucleases created
by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the Fok I
endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand
breaks to specific, targeted sites.
targeted mutagenesis. Here we report a new class of sequence-specific nucleases created
by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the Fok I
endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand
breaks to specific, targeted sites.
Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
Oxford University Press