Abstract Two‐photon (2P) microscopy is a high‐resolution imaging technique that was
initially applied by neurobiologists and developmental cell biologists but has subsequently
been broadly adapted by immunologists. The value of 2P microscopy is that it affords an
unparalleled view of single‐cell spatiotemporal dynamics deep within intact tissues and
organs. As the technology develops and new transgenic mice and fluorescent probes
become available, 2P microscopy will serve as an increasingly valuable tool for assessing …