[HTML][HTML] Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system
Biochemical and biophysical research communications, 2014•Elsevier
The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted
genome-modified animals. However, this system is limited by the risk of off-target mutations.
Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate
region deleted mice and targeted knock-in mice without off-target mutations.
genome-modified animals. However, this system is limited by the risk of off-target mutations.
Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate
region deleted mice and targeted knock-in mice without off-target mutations.
Abstract
The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice without off-target mutations.
Elsevier