Transgenic expression of cholesterol 7α‐hydroxylase in the liver prevents high‐fat diet–induced obesity and insulin resistance in mice
Materials and Methods Animals. Cyp7a1-tg mice overexpressing rat Cyp7a1 complementary
DNA under the control of an apolipoprotein E3 (ApoE3) hepatic control region were
originally generated by Dr. Roger A. Davis (San Diego State University) with a C57BL/6JxSJ
strain and were backcrossed into a C57BL/6J background for five generations in the original
laboratory. 15 We obtained the Cyp7a1-tg mice from the Mutant Mouse Regional Resource
Center at the University of California Davis [strain name: B6. Cg-Tg (APOE-Cyp7a1) …
DNA under the control of an apolipoprotein E3 (ApoE3) hepatic control region were
originally generated by Dr. Roger A. Davis (San Diego State University) with a C57BL/6JxSJ
strain and were backcrossed into a C57BL/6J background for five generations in the original
laboratory. 15 We obtained the Cyp7a1-tg mice from the Mutant Mouse Regional Resource
Center at the University of California Davis [strain name: B6. Cg-Tg (APOE-Cyp7a1) …