[HTML][HTML] Identification of novel TGF-β/Smad gene targets in dermal fibroblasts using a combined cDNA microarray/promoter transactivation approach
F Verrecchia, ML Chu, A Mauviel - Journal of biological chemistry, 2001 - ASBMB
Despite major advances in the understanding of the intimate mechanisms of transforming
growth factor-β (TGF-β) signaling through the Smad pathway, little progress has been made
in the identification of direct target genes. In this report, using cDNA microarrays, we have
focussed our attention on the characterization of extracellular matrix-related genes rapidly
induced by TGF-β in human dermal fibroblasts and attempted to identify the ones whose up-
regulation by TGF-β is Smad-mediated. For a gene to qualify as a direct Smad target, we …
growth factor-β (TGF-β) signaling through the Smad pathway, little progress has been made
in the identification of direct target genes. In this report, using cDNA microarrays, we have
focussed our attention on the characterization of extracellular matrix-related genes rapidly
induced by TGF-β in human dermal fibroblasts and attempted to identify the ones whose up-
regulation by TGF-β is Smad-mediated. For a gene to qualify as a direct Smad target, we …