[HTML][HTML] Proteomic analysis demonstrates activator-and chromatin-specific recruitment to promoters

TW Sikorski, YJ Joo, SB Ficarro, M Askenazi… - Journal of Biological …, 2012 - ASBMB
In-depth characterization of RNA polymerase II preinitiation complexes remains an important
and challenging goal. We used quantitative mass spectrometry to explore context-
dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter
reconstituted on naked and chromatinized DNA templates. The transcription activators Gal4-
VP16 and Gal4-Gcn4 recruited a limited set of chromatin-related coactivator complexes,
namely the chromatin remodeler Swi/Snf and histone acetyltransferases SAGA and NuA4 …