Detection of functional antigen-specific T cells from urine of non-muscle invasive bladder cancer patients

C Pieraerts, V Martin, P Jichlinski… - …, 2012 - Taylor & Francis
C Pieraerts, V Martin, P Jichlinski, D Nardelli-Haefliger, L Derré
Oncoimmunology, 2012Taylor & Francis
To gain further insights into the role of T lymphocytes in immune responses against bladder
tumors, we developed a method that monitors the presence of functional antigen-specific T
cells in the urine of non-muscle invasive bladder cancer patients. As relatively few immune
cells can usually be recovered from urine, we examined different isolation/amplification
protocols and took advantage of patients treated with weekly intravesical instillations of
Bacillus Calmette-Guérin, resulting in large amounts of immune cells into urine. Our findings …
To gain further insights into the role of T lymphocytes in immune responses against bladder tumors, we developed a method that monitors the presence of functional antigen-specific T cells in the urine of non-muscle invasive bladder cancer patients. As relatively few immune cells can usually be recovered from urine, we examined different isolation/amplification protocols and took advantage of patients treated with weekly intravesical instillations of Bacillus Calmette-Guérin, resulting in large amounts of immune cells into urine. Our findings demonstrate that, upon in vitro amplification, antigen-specific T cells can be detected by an interferon γ (IFNγ)-specific ELISPOT assay.
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