Age-related differences in CYP3A expression and activity in the rat liver, intestine, and kidney
JS Warrington, DJ Greenblatt, LL von Moltke - The Journal of pharmacology …, 2004 - Elsevier
We evaluated the effect of age on CYP3A expression and function in the liver, intestine, and
kidney from young (3-4 months), intermediate (13-14 months), and old (25-26 months) male
Fischer-344 rats. The biotransformation of triazolam to its primary hydroxylated products, 4-
OH-TRZ (triazolam) and α-OH-TRZ, was used as a marker of CYP3A activity in rat liver and
intestine. Immunoactive CYP3A expression was evaluated by Western blot analysis in the
rat intestine, liver, and kidney. Since testosterone and NADPH reductase levels may …
kidney from young (3-4 months), intermediate (13-14 months), and old (25-26 months) male
Fischer-344 rats. The biotransformation of triazolam to its primary hydroxylated products, 4-
OH-TRZ (triazolam) and α-OH-TRZ, was used as a marker of CYP3A activity in rat liver and
intestine. Immunoactive CYP3A expression was evaluated by Western blot analysis in the
rat intestine, liver, and kidney. Since testosterone and NADPH reductase levels may …
