Apoptotic retinal ganglion cell death in the DBA/2 mouse model of glaucoma

D Reichstein, L Ren, T Filippopoulos, T Mittag… - Experimental eye …, 2007 - Elsevier
The DBA/2 mouse has been used as a model for spontaneous secondary glaucoma. We
attempted to determine the in vivo time course and spatial distribution of retinal ganglion
cells (RGCs) undergoing apoptotic death in DBA/2 mice. Female DBA/2 mice, 3, 9–10, 12,
15, and 18 months of age, received intravitreal injections of Annexin-V conjugated to
AlexaFluor 1h prior to euthanasia. Retinas were fixed and flat-mounted. Annexin-V-positive
RGCs in the hemiretina opposite the site of injection were counted, and their locations were …