The advent of rapid evolution on sequencing capacity of new genomes has evidenced the need for data analysis automation aiming at speeding up the genomic annotation process and reducing its cost. Given that one important step for functional genomic annotation is the promoter identification, several studies have been taken in order to propose computational approaches to predict promoters. Different classifiers and characteristics of the promoter sequences have been used to deal with this prediction problem. However, several works in literature have addressed the promoter prediction problem using datasets containing sequences of 250 nucleotides or more. As the sequence length defines the amount of dataset attributes, even considering a limited number of properties to characterize the sequences, datasets with a high number of attributes are generated for training classifiers. Once high-dimensional datasets can degrade the classifiers predictive performance or even require an infeasible processing time, predicting promoters by training classifiers from datasets with a reduced number of attributes, it is essential to obtain good predictive performance with low computational cost. To the best of our knowledge, there is no work in literature that verified in a systematic way the relation between the sequences length and the predictive performance of classifiers. Thus, in this work, we have evaluated the impact of sequence length variation and training dataset size (number of sequences) on the predictive performance of classifiers.

We have built sixteen datasets composed of different sized sequences (ranging in length from 12 to 301 nucleotides) and evaluated them using the SVM, Random Forest and k-NN classifiers. The best predictive performances reached by SVM and Random Forest remained relatively stable for datasets composed of sequences varying in length from 301 to 41 nucleotides, while k-NN achieved its best performance for the dataset composed of 101 nucleotides. We have also analyzed, using sequences composed of only 41 nucleotides, the impact of increasing the number of sequences in a dataset on the predictive performance of the same three classifiers. Datasets containing 14,000, 80,000, 100,000 and 120,000 sequences were built and evaluated. All classifiers achieved better predictive performance for datasets containing 80,000 sequences or more.

The experimental results show that several datasets composed of shorter sequences achieved better predictive performance when compared with datasets composed of longer sequences, and also consumed a significantly shorter processing time. Furthermore, increasing the number of sequences in a dataset proved to be beneficial to the predictive power of classifiers.