Whereas high shearing flows are known to induce endothelial cell remodeling, we show
here that very low interstitial flow rates trigger endothelial cell morphogenesis in 3D cultures.
Interstitial flow is a functionally critical component of the circulation, and we have recently
observed that it plays a regulatory role in lymphangiogenesis; here we investigate interstitial
flow as a powerful morphoregulatory stimulant. We exposed both lymphatic and blood
endothelial cells (LECs and BECs) to interstitial flow in 3D collagen gels as well as simple …