Caspase activation during apoptotic cell death induced by expanded polyglutamine in N2a cells
G Wang, K Mitsui, S Kotliarova, A Yamashita… - …, 1999 - journals.lww.com
HUNTINGTON disease (HD) is an autosomal dominant neurodegenerative disorder. To
investigate the mechanism of neurodegeneration induced by mutant huntingtin, we
developed a stable neuro2a cell line expressing truncated N-terminal huntingtin (tNhtt) with
EGFP using the ecdysone-inducible system. The formation of aggregates and the cell death
induced by expression of tNhtt with expanded polyglutamine was repeat length and dose-
dependent. Caspases were activated, and the death substrates of caspases, lamin B and …
investigate the mechanism of neurodegeneration induced by mutant huntingtin, we
developed a stable neuro2a cell line expressing truncated N-terminal huntingtin (tNhtt) with
EGFP using the ecdysone-inducible system. The formation of aggregates and the cell death
induced by expression of tNhtt with expanded polyglutamine was repeat length and dose-
dependent. Caspases were activated, and the death substrates of caspases, lamin B and …