[HTML][HTML] Two Vitamin D Response Elements Function in the Rat 1, 25-Dihydroxyvitamin D 24-Hydroxylase Promoter (∗)

C Zierold, HM Darwish, HF DeLuca - Journal of Biological Chemistry, 1995 - ASBMB
The interaction between the two vitamin D response elements (DRE) located at− 154 to−
134 base pairs (bp) and− 262 to− 238 bp from the transcription initiation site has been
studied using reporter gene assays and binding assays by electrophoretic gel shift
measurements. 3 half-sites separated by 3 bp were found necessary for transactivation by
the− 154 to− 125 DRE, while 2 half-sites separated by 3 bp were needed for the DRE at−
262 to− 238 to function. However, the two DREs together provided maximal activity. The 93 …