A Specific High-Affinity Binding Macromolecule for 1,25-Dihydroxyvitamin D3 in Fetal Bone
BE Kream, M Jose, S Yamada, HF DeLuca - Science, 1977 - science.org
BE Kream, M Jose, S Yamada, HF DeLuca
Science, 1977•science.orgCytosol fractions were prepared from fetal rat or embryonic chick calvaria and analyzed for
binding of vitamin D3 metabolites on sucrose density gradients. Both cytosol fractions
contain a 3.5 S macromolecule which specifically binds 1, 25-dihydroxy-[3H] vitamin D3 and
in addition, a 5 to 6 S macromolecule which binds 25-hydroxy-[3H] vitamin D3. In rat calvaria
cytosol, 1, 25-dihydroxy-[3H] vitamin D3 also binds to the 5 to 6 S macromolecule but
appears to have greater affinity for the 3.5 S component.
binding of vitamin D3 metabolites on sucrose density gradients. Both cytosol fractions
contain a 3.5 S macromolecule which specifically binds 1, 25-dihydroxy-[3H] vitamin D3 and
in addition, a 5 to 6 S macromolecule which binds 25-hydroxy-[3H] vitamin D3. In rat calvaria
cytosol, 1, 25-dihydroxy-[3H] vitamin D3 also binds to the 5 to 6 S macromolecule but
appears to have greater affinity for the 3.5 S component.
Cytosol fractions were prepared from fetal rat or embryonic chick calvaria and analyzed for binding of vitamin D3 metabolites on sucrose density gradients. Both cytosol fractions contain a 3.5S macromolecule which specifically binds 1,25-dihydroxy-[3H]vitamin D3 and in addition, a 5 to 6S macromolecule which binds 25-hydroxy-[3H]vitamin D3. In rat calvaria cytosol, 1,25-dihydroxy-[3H]vitamin D3 also binds to the 5 to 6S macromolecule but appears to have greater affinity for the 3.5S component.
AAAS