[HTML][HTML] Molecular genetics and phenotypic characteristics of MODY caused by hepatocyte nuclear factor 4α mutations in a large European collection

ER Pearson, S Pruhova, CJ Tack, A Johansen… - Diabetologia, 2005 - Springer
ER Pearson, S Pruhova, CJ Tack, A Johansen, HAJ Castleden, PJ Lumb, AS Wierzbicki
Diabetologia, 2005Springer
Aims/hypothesis Heterozygous mutations in the gene of the transcription factor hepatocyte
nuclear factor 4α (HNF-4α) are considered a rare cause of MODY with only 14 mutations
reported to date. The description of the phenotype is limited to single families. We
investigated the genetics and phenotype of HNF-4α mutations in a large European
Caucasian collection. Methods HNF-4α was sequenced in 48 MODY probands, selected for
a phenotype of HNF-1α MODY but negative for HNF-1α mutations. Clinical characteristics …
Aims/hypothesis
Heterozygous mutations in the gene of the transcription factor hepatocyte nuclear factor 4α (HNF-4α) are considered a rare cause of MODY with only 14 mutations reported to date. The description of the phenotype is limited to single families. We investigated the genetics and phenotype of HNF-4α mutations in a large European Caucasian collection.
Methods
HNF-4α was sequenced in 48 MODY probands, selected for a phenotype of HNF-1α MODY but negative for HNF-1α mutations. Clinical characteristics and biochemistry were compared between 54 HNF-4α mutation carriers and 32 familial controls from ten newly detected or previously described families.
Results
Mutations in HNF-4α were found in 14/48 (29%) probands negative for HNF-1α mutations. The mutations found included seven novel mutations: S34X, D206Y, E276D, L332P, I314F, L332insCTG and IVS5nt+1G>A. I314F is the first reported de novo HNF-4α mutation. The average age of diagnosis was 22.9 years with frequent clinical evidence of sensitivity to sulphonylureas. Beta cell function, but not insulin sensitivity, was reduced in diabetic mutation carriers compared to control subjects (homeostasis model assessment of beta cell function 29% p<0.001 vs controls). HNF-4α mutations were associated with lower apolipoprotein A2 (p=0.001), A1 (p=0.04) and total HDL-cholesterol (p=0.02) than in control subjects. However, in contrast to some previous reports, levels of triglycerides and apolipoprotein C3 were normal.
Conclusions/interpretation
HNF-4α mutations are common when no HNF-1α mutation is found in strictly defined MODY families. The HNF-4α clinical phenotype and beta cell dysfunction are similar to HNF-1α MODY and are associated with reduced apolipoprotein A2 levels. We suggest that sequencing of HNF-4α should be performed in patients with clinical characteristics of HNF-1α MODY in whom mutations in HNF-1α are not found.
Springer