[HTML][HTML] Optimization of a murine immunization model for study of PF4/heparin antibodies
S Suvarna, R Qi, GM Arepally - Journal of Thrombosis and Haemostasis, 2009 - Elsevier
Background: Heparin-induced thrombocytopenia (HIT) is a life-threatening thrombotic illness
caused by drug-dependent antibodies recognizing complexes of platelet factor 4 (PF4) and
heparin. Little is known about the immune pathogenesis of HIT, in particular factors
influencing PF4/heparin antibody formation. To gain insight into the biologic basis of heparin
sensitization, we have recently developed an animal model using wild-type (WT) mice in
which murine PF4/heparin antibodies (anti-mPF4/H) arise de novo after antigen challenge …
caused by drug-dependent antibodies recognizing complexes of platelet factor 4 (PF4) and
heparin. Little is known about the immune pathogenesis of HIT, in particular factors
influencing PF4/heparin antibody formation. To gain insight into the biologic basis of heparin
sensitization, we have recently developed an animal model using wild-type (WT) mice in
which murine PF4/heparin antibodies (anti-mPF4/H) arise de novo after antigen challenge …