[HTML][HTML] Contributions of the LG modules and furin processing to laminin-2 functions
SP Smirnov, EL McDearmon, S Li, JM Ervasti… - Journal of Biological …, 2002 - ASBMB
The α2-laminin subunit contributes to basement membrane functions in muscle, nerve, and
other tissues, and mutations in its gene are causes of congenital muscular dystrophy. The α2
G-domain modules, mutated in several of these disorders, are thought to mediate different
cellular interactions. To analyze these contributions, we expressed recombinant laminin-2 (α
2 β 1 γ 1) with LG4–5, LG1–3, and LG1–5 modular deletions. Wild-type and LG4–5 deleted-
laminins were isolated from medium intact and cleaved within LG3 by a furin-like …
other tissues, and mutations in its gene are causes of congenital muscular dystrophy. The α2
G-domain modules, mutated in several of these disorders, are thought to mediate different
cellular interactions. To analyze these contributions, we expressed recombinant laminin-2 (α
2 β 1 γ 1) with LG4–5, LG1–3, and LG1–5 modular deletions. Wild-type and LG4–5 deleted-
laminins were isolated from medium intact and cleaved within LG3 by a furin-like …