[HTML][HTML] Heterogeneous processing and zona pellucida binding activity of pig zonadhesin

JR Hickox, M Bi, DM Hardy - Journal of Biological Chemistry, 2001 - ASBMB
Zonadhesin is a mosaic protein in sperm membrane fractions that binds directly and in a
species-specific manner to the extracellular matrix (zona pellucida) of the oocyte. The active
form of pig zonadhesin from capacitated, epididymal spermatozoa comprises two covalently
associated polypeptide chains of M r 105,000 (p105) and M r 45,000 (p45). Here we report
detection and characterization of multiple zonadhesin isoforms in freshly ejaculated cells.
Antibodies to the predicted von Willebrand D0-D1, D1, and D3 domains of pig zonadhesin …