Basic image analysis and manipulation in ImageJ

SM Hartig - Current protocols in molecular biology, 2013 - Wiley Online Library
Current protocols in molecular biology, 2013Wiley Online Library
Image analysis methods have been developed to provide quantitative assessment of
microscopy data. In this unit, basic aspects of image analysis are outlined, including
software installation, data import, image processing functions, and analytical tools that can
be used to extract information from microscopy data using ImageJ. Step‐by‐step protocols
for analyzing objects in a fluorescence image and extracting information from two‐color
tissue images collected by bright‐field microscopy are included. Curr. Protoc. Mol. Biol. 102 …
Abstract
Image analysis methods have been developed to provide quantitative assessment of microscopy data. In this unit, basic aspects of image analysis are outlined, including software installation, data import, image processing functions, and analytical tools that can be used to extract information from microscopy data using ImageJ. Step‐by‐step protocols for analyzing objects in a fluorescence image and extracting information from two‐color tissue images collected by bright‐field microscopy are included. Curr. Protoc. Mol. Biol. 102:14.15.1–14.15.12. © 2013 by John Wiley & Sons, Inc.
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