Epidermal growth factor induces the tyrosine phosphorylation and nuclear translocation of Stat 5 in mouse liver.

S Ruff-Jamison, K Chen… - Proceedings of the …, 1995 - National Acad Sciences
S Ruff-Jamison, K Chen, S Cohen
Proceedings of the National Academy of Sciences, 1995National Acad Sciences
Intraperitoneal injection of epidermal growth factor into mice results in the appearance of
multiple tyrosine-phosphorylated proteins in liver nuclei within minutes after administration.
We have previously identified three of these proteins as Stat 1 alpha, Stat 1 beta (p91, p84),
and Stat 3 (p89). In the present report we demonstrate that Stat 5 (p92), the recently
described prolactin inducible transcription factor detected in mammary glands, is the major
tyrosine-phosphorylated protein translocated to the nucleus in mouse liver in response to …
Intraperitoneal injection of epidermal growth factor into mice results in the appearance of multiple tyrosine-phosphorylated proteins in liver nuclei within minutes after administration. We have previously identified three of these proteins as Stat 1 alpha, Stat 1 beta (p91, p84), and Stat 3 (p89). In the present report we demonstrate that Stat 5 (p92), the recently described prolactin inducible transcription factor detected in mammary glands, is the major tyrosine-phosphorylated protein translocated to the nucleus in mouse liver in response to epidermal growth factor. Furthermore, gel-shift analysis and affinity purification revealed that Stat 5, Stat 1 alpha, and Stat 1 beta specifically bind to the prolactin inducible element upstream of the beta-casein promoter.
National Acad Sciences