[HTML][HTML] Requirement for the Synergy Site for Cell Adhesion to Fibronectin Depends on the Activation State of Integrin α5β1 (∗)

EHJ Danen, S Aota, AA van Kraats, KM Yamada… - Journal of Biological …, 1995 - ASBMB
We investigated the influence of the activation state of integrin α5β1 on its dependence on
the PHSRN synergy site for binding to RGD in fibronectin. K562 and MV3 cells lacked αvβ3
expression and adhered to fibronectin through α5β1. Mel57 cells adhered through αvβ3 and
α5β1. A recombinant fibronectin polypeptide, containing five type III repeats from the central
cell binding domain 3Fn6-10, and a mutated polypeptide lacking the synergy site were
equally effective in promoting Mel57 adhesion. For K562 and MV3, the mutated polypeptide …