[HTML][HTML] Mast cells infiltrate pancreatic islets in human type 1 diabetes
L Martino, M Masini, M Bugliani, L Marselli, M Suleiman… - Diabetologia, 2015 - Springer
L Martino, M Masini, M Bugliani, L Marselli, M Suleiman, U Boggi, TC Nogueira, F Filipponi…
Diabetologia, 2015•SpringerMethods Pancreatic tissue from donors without diabetes and with type 1 and 2 diabetes was
studied using different microscopy techniques to identify islet-infiltrating cells. The direct
effects of histamine exposure on isolated human islets and INS-1E cells were assessed
using cell-survival studies and molecular mechanisms. Results A larger number of mast
cells were found to infiltrate pancreatic islets in samples from donors with type 1 diabetes,
compared with those from donors without diabetes or with type 2 diabetes. Evidence of mast …
studied using different microscopy techniques to identify islet-infiltrating cells. The direct
effects of histamine exposure on isolated human islets and INS-1E cells were assessed
using cell-survival studies and molecular mechanisms. Results A larger number of mast
cells were found to infiltrate pancreatic islets in samples from donors with type 1 diabetes,
compared with those from donors without diabetes or with type 2 diabetes. Evidence of mast …
Methods
Pancreatic tissue from donors without diabetes and with type 1 and 2 diabetes was studied using different microscopy techniques to identify islet-infiltrating cells. The direct effects of histamine exposure on isolated human islets and INS-1E cells were assessed using cell-survival studies and molecular mechanisms.
Results
A larger number of mast cells were found to infiltrate pancreatic islets in samples from donors with type 1 diabetes, compared with those from donors without diabetes or with type 2 diabetes. Evidence of mast cell degranulation was observed, and the extent of the infiltration correlated with beta cell damage. Histamine, an amine that is found at high levels in mast cells, directly contributed to beta cell death in isolated human islets and INS-1E cells via a caspase-independent pathway.
Springer