Characterization of muscarinic receptor subtype of rat eccrine sweat gland by autoradiography
NE Torres, PJ Zollman, PA Low - Brain research, 1991 - Elsevier
NE Torres, PJ Zollman, PA Low
Brain research, 1991•ElsevierThe muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using
quantitative autoradiography snd [3 H] QNB as radioligand. The distribution of radioligand
was maximal in the secretory coil. Autoradiographic competition binding sites studies were
performed using selective antagonists to M 1 (pirenzepine), M 2 (AF-DX 116), and M 3 (4-
DAMP) and the classical nonselective antagonist atropine. pK i for pirenzepine, AF-DX 116,
4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine …
quantitative autoradiography snd [3 H] QNB as radioligand. The distribution of radioligand
was maximal in the secretory coil. Autoradiographic competition binding sites studies were
performed using selective antagonists to M 1 (pirenzepine), M 2 (AF-DX 116), and M 3 (4-
DAMP) and the classical nonselective antagonist atropine. pK i for pirenzepine, AF-DX 116,
4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine …
Abstract
The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography snd [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding sites studies were performed using selective antagonists to M1 (pirenzepine), M2 (AF-DX 116), and M3 (4-DAMP) and the classical nonselective antagonist atropine.pKi for pirenzepine, AF-DX 116, 4-DAMP, and atropine was 6.58, 5.47, 8.50, and 8.66 respectively indicating that the eccrine sweat gland muscarinic receptor was predominantly M3.
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