Intracellular pH Regulation in Cultured Astrocytes from Rat Hippocampus: II. Electrogenic Na/HCO3 Cotransport

MO Bevensee, M Apkon, WF Boron - The Journal of general physiology, 1997 - rupress.org
MO Bevensee, M Apkon, WF Boron
The Journal of general physiology, 1997rupress.org
In the preceding paper (Bevensee, MO, RA Weed, and WF Boron. 1997. J. Gen. Physiol.
110: 453–465.), we showed that a Na+-driven influx of HCO3− causes the increase in
intracellular pH (pHi) observed when astrocytes cultured from rat hippocampus are exposed
to 5% CO2/17 mM HCO3−. In the present study, we used the pH-sensitive fluorescent
indicator 2′, 7′-biscarboxyethyl-5, 6-carboxyfluorescein (BCECF) and the perforated
patch-clamp technique to determine whether this transporter is a Na+-driven Cl-HCO3 …
In the preceding paper (Bevensee, M.O., R.A. Weed, and W.F. Boron. 1997. J. Gen. Physiol. 110: 453–465.), we showed that a Na+-driven influx of HCO3 causes the increase in intracellular pH (pHi) observed when astrocytes cultured from rat hippocampus are exposed to 5% CO2/17 mM HCO3. In the present study, we used the pH-sensitive fluorescent indicator 2′,7′-biscarboxyethyl-5,6-carboxyfluorescein (BCECF) and the perforated patch-clamp technique to determine whether this transporter is a Na+-driven Cl-HCO3 exchanger, an electrogenic Na/HCO3 cotransporter, or an electroneutral Na/HCO3 cotransporter. To determine if the transporter is a Na+-driven Cl-HCO3 exchanger, we depleted the cells of intracellular Cl by incubating them in a Cl-free solution for an average of ∼11 min. We verified the depletion with the Cl-sensitive dye N-(6-methoxyquinolyl)acetoethyl ester (MQAE). In Cl-depleted cells, the pHi still increases after one or more exposures to CO2/HCO3. Furthermore, the pHi decrease elicited by external Na+ removal does not require external Cl. Therefore, the transporter cannot be a Na+-driven Cl-HCO3 exchanger. To determine if the transporter is an electrogenic Na/ HCO3 cotransporter, we measured pHi and plasma membrane voltage (Vm) while removing external Na+, in the presence/absence of CO2/HCO3 and in the presence/absence of 400 μM 4,4′-diisothiocyanatostilbene-2,2′-disulphonic acid (DIDS). The CO2/HCO3 solutions contained 20% CO2 and 68 mM HCO3, pH 7.3, to maximize the HCO3 flux. In pHi experiments, removing external Na+ in the presence of CO2/HCO3 elicited an equivalent HCO3 efflux of 281 μM s−1. The HCO3 influx elicited by returning external Na+ was inhibited 63% by DIDS, so that the predicted DIDS-sensitive Vm change was 3.3 mV. Indeed, we found that removing external Na+ elicited a DIDS-sensitive depolarization that was 2.6 mV larger in the presence than in the absence of CO2/ HCO3. Thus, the Na/HCO3 cotransporter is electrogenic. Because a cotransporter with a Na+:HCO3 stoichiometry of 1:3 or higher would predict a net HCO3 efflux, rather than the required influx, we conclude that rat hippocampal astrocytes have an electrogenic Na/HCO3 cotransporter with a stoichiometry of 1:2.
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