[HTML][HTML] Tmem16A encodes the Ca2+-activated Cl− channel in mouse submandibular salivary gland acinar cells

VG Romanenko, MA Catalán, DA Brown… - Journal of Biological …, 2010 - ASBMB
Activation of an apical Ca 2+-dependent Cl− channel (CaCC) is the rate-limiting step for fluid
secretion in many exocrine tissues. Here, we compared the properties of native CaCC in
mouse submandibular salivary gland acinar cells to the Ca 2+-gated Cl− currents generated
by Tmem16A and Best2, members from two distinct families of Ca 2+-activated Cl− channels
found in salivary glands. Heterologous expression of Tmem16A and Best2 transcripts in
HEK293 cells produced Ca 2+-activated Cl− currents with time and voltage dependence …