[HTML][HTML] Generation of human pulmonary microvascular endothelial cell lines
V Krump-Konvalinkova, F Bittinger, RE Unger… - Laboratory …, 2001 - Elsevier
The limited lifespan of human microvascular endothelial cells in cell culture represents a
major obstacle for the study of microvascular pathobiology. To date, no endothelial cell line
is available that demonstrates all of the fundamental characteristics of microvascular
endothelial cells. We have generated endothelial cell lines from human pulmonary
microvascular endothelial cells (HPMEC) isolated from adult donors. HPMEC were
cotransfected with a plasmid encoding the catalytic component of telomerase (hTERT) and a …
major obstacle for the study of microvascular pathobiology. To date, no endothelial cell line
is available that demonstrates all of the fundamental characteristics of microvascular
endothelial cells. We have generated endothelial cell lines from human pulmonary
microvascular endothelial cells (HPMEC) isolated from adult donors. HPMEC were
cotransfected with a plasmid encoding the catalytic component of telomerase (hTERT) and a …